Part:BBa_K1446002
superfolder GFP with N-terminal pdu-microcompartment tag
We have used superfolded GFP with the N-terminal pdu-microcompartment tag in order to get fluorescence images that would show localised spots of higher GFP-density, indicating that the microcompartment tag successfully inserts the attached protein into the microcompartments
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 91
To test the targeting of the N-terminal tag, we have used Fluorescence microscopy on cells that have been transformed with two vectors, one containing this part and one containing the microcompartment submitted by the iGEM Dundee team in 2009 ( BBa_K562009 ). As can be seen for some of the cells in the image below, there is no perfect and uniform distribution of sfGFP, but clearly identifiable spots of higher fluorescence densities which suggests that the targeting mechanism of the N-terminal tag has worked successfully.
For experimental details, see the experience page for this part.
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